Multiplex PCR to Differentiate Monkeypox Virus Clades.
Williams CT., Romero-Ramirez A., Semiu AA., Ifabumuyi SO., Greenland-Bews C., Gould S., Wooding D., Allen C., Somasundaran A., Mkpuma NN., Gado D., Adole JA., Amoo AE., Adeyemi AA., de Jarcy LB., Goffinet C., Dunning J., Semple MG., Bahizire E., Akinpelu A., Fletcher TE., Cubas-Atienzar AI., Leggio C., Adedeji A., Omoare AA., Edwards T., International Severe Acute Respiratory and Emerging Infection Consortium Investigators .
We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%-86.14%) and specificity 94% (95% CI 80.84%-99.30%); for samples with cycle thresholds <35, sensitivity was 98% (95% CI 91.72%-99.96%) and specificity 94% (95% CI 80.84%-99.30%).