Electron fourier ptychography for phase reconstruction.

Zhao J., Huang C., Mostaed A., Moshtaghpour A., Parkhurst JM., Lobato I., Gallagher-Jones M., Kim JS., Boyce M., Stuart D., Andreeva EA., Colletier J-P., Kirkland AI.

Exit wavefunction reconstruction is important in transmission electron microscopy for structural studies. We describe electron Fourier ptychography and its application to phase reconstruction of both radiation-resistant and beam-sensitive materials. We demonstrate that the phase of the exit wave can be reconstructed to high resolution using a modified iterative phase retrieval algorithm from data collected in an alternative optical geometry. This method achieves a spatial resolution of 0.63 nm at a fluence of 4.5 × 102 e-/nm2, as validated on Cry11Aa protein crystals under cryogenic conditions. Notably, this method requires no instrumental modifications, is straightforward to implement, and can be seamlessly integrated with existing data collection software, providing a broadly accessible alternative approach for structural studies.

DOI

10.1038/s41598-025-21638-7

Type

Journal article

Publication Date

2025-10-30T00:00:00+00:00

Volume

15

Keywords

Cryogenic condition, Electron Fourier ptychography, Low fluence, Phase reconstruction

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